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The function of a vector in genetic engineering is to


A) cut DNA into many fragments.
B) introduce rDNA into a host cell.
C) link together newly joined fragments of DNA.
D) make millions of copies of a specific segment of DNA.
E) separate fragments of DNA by their length and electrical charges.

F) C) and D)
G) A) and C)

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Biotechnology products produced by bacteria include all of the following EXCEPT


A) antibodies to deliver radioisotopes to tumor cells.
B) clotting factor VIII.
C) human growth hormone.
D) insulin.

E) B) and C)
F) All of the above

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Which of the following statements about RNAi are true? Select all that apply.


A) RNAi sequences are designed to be complementary to the rRNA that will function in the translation of a gene of interest.
B) Once the complementary RNAi sequences enter the cell,they bind with the target RNA,producing double-stranded RNA molecules.
C) These double-stranded RNA molecules are then broken down by a series of enzymes within the cell.
D) RNAi is being researched as a potential treatment for a number of human diseases including cancer and hepatitis.

E) All of the above
F) A) and B)

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Which of the following is mismatched?


A) bioinformatics-the study of genomic and proteomic information using computer analysis
B) polymerase chain reaction-process that separates DNA fragments according to size
C) genomics-the study of the genomes of humans and other organisms
D) proteomics-the study of species' proteins

E) C) and D)
F) None of the above

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When using bacteria to clone a human gene and express its product,as in the case of insulin,the gene cannot contain introns because bacteria do not have the enzymes to process mRNA.How do scientists overcome this challenge?

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Reverse transcriptase is used to "revers...

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Place the following steps for cloning DNA in the correct order: 1 - use vector to deliver new rDNA to bacterial or other cells 2 - use restriction enzymes to cut a plasmid and add the desired gene 3 - isolate and cut out a desired gene using restriction enzymes 4 - use DNA ligase to seal the new gene 5 - allow bacterial cells to replicate and produce desired product


A) 1,2,3,4,5
B) 5,3,2,4,1
C) 2,5,3,1,4
D) 3,2,4,1,5

E) A) and B)
F) B) and D)

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What is the function of gel electrophoresis in genetic engineering?


A) cut DNA into many fragments
B) carry DNA into a new cell
C) link together newly joined fragments of DNA
D) make millions of copies of a specific segment of DNA
E) separate fragments of DNA by their length and electrical charges

F) C) and D)
G) A) and E)

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Which of the following molecules forms lengths of DNA with "sticky ends"?


A) DNA ligase
B) DNA polymerase
C) RNA polymerase
D) reverse transcriptase
E) restriction enzyme

F) A) and C)
G) B) and C)

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A transgenic organism


A) contains a foreign gene within its genome.
B) is produced by cloning a mutant cell.
C) acts as the vector for DNA being moved into another organism.
D) is produced by the polymerase chain reaction.
E) is any genetically modified organism resulting from laboratory research.

F) B) and E)
G) B) and C)

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The term "ligase" adds the suffix "-ase" to the Latin root word for


A) gene.
B) work.
C) cut.
D) bind.
E) copy-producing.

F) A) and B)
G) B) and D)

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What is the difference between ex vivo and in vivo gene therapy?

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In ex vivo gene therapy,tissue such as b...

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What consequences could arise if the field of bioinformatics ceased to exist as a field of study?


A) Progress in determining the function of DNA sequences,making comparisons between our genome and other model organisms,and understanding how genes and proteins interact with cells would stop.
B) Many cures for genetic disorders would not be discovered.
C) The creation of new transgenic plants,animals,and bacteria that benefit society would not occur.
D) All of these are consequences if the field of bioinformatics ceased to exist as a field of study.

E) None of the above
F) All of the above

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