Question 1

What method must be performed to produce enough DNA for sequencing?

Accepted Answer

A)   PCR
B)   Southern blotting
C)   microarray analyses
D)   Western blotting
E)   Northern blottingF) A) and B)
G) C) and D)

Question 2

A plasmid vector and chromosomal DNA are treated separately with the same restriction enzyme. Which of the following might occur if the digested plasmid and chromosomal DNA were incubated together?

Accepted Answer

A)   The two sticky ends of the plasmid could hybridize back together, recircularizing.
B)   The two sticky ends of the plasmid could hybridize to both sticky ends of the chromosomal DNA fragments.
C)   The two sticky ends of the plasmid could hybridize back together and recircularize as well as
D)   The two sticky ends of the plasmid could hybridize with two ends of another type of plasmid.
E)   The two sticky ends of the vector would remain free or unhybridized.F) A) and B)
G) D) and E)

Question 3

Which of the following sequence pairs is a palindrome?

Accepted Answer

A)   5?-GTTCAT-3?; 3?-CAAGTA-5?
B)   5?-GAATCG-3?; 3?-CTTAGC-5?
C)   5?-TCCGGA-3?; 3?-AGGCCT-5?
D)   5?-TCACGT-3?; 3?-TCACGT-5?
E)   None of the paired sequences are a palindrome.F) C) and D)
G) A) and E)

Question 4

A researcher wants to clone a specific gene of interest. Why would he/she choose a viral vector for introducing the gene of interest into a host cell?

Accepted Answer

A)   A viral vector contains its own metabolic machinery and does not affect the host cell.
B)   A viral vector can infect living cells and take control of the host cell's metabolic machinery.
C)   A viral vector is nonliving.
D)   A viral vector contains circular DNA, thus it is easier to insert the gene of interest.
E)   None of the choices are correct.F) B) and E)
G) A) and C)

Question 5

Which of the following acts as a carrier of foreign DNA and is needed to clone a gene?

Accepted Answer

A)   plasmid
B)   viral vector
C)   fungal vector
D)   plasmid and viral vector
E)   viral vector and fungal vectorF) A) and E)
G) None of the above

Question 6

What is the purpose of a selectable marker such as the antibiotic-resistance gene ampR?

Accepted Answer

A)   Selectable markers allow a researcher to identify cells that have incorporated the recombinant plasmid.
B)   Selectable markers produce antibiotics, which kill cells that have not incorporated recombinant plasmid.
C)   Selectable markers prevent cells that have incorporated one plasmid from incorporating another.
D)   Selectable markers allow a researcher to identify the recombinant plasmid.
E)   Selectable markers are only effective in viral vectors.F) A) and D)
G) B) and E)

Question 7

Under what circumstances would a molecular geneticist need to use a bacterial artificial chromosome (BAC) ?

Accepted Answer

A)   when cloning small, prokaryotic genomes
B)   when cloning small, eukaryotic genomes
C)   when cloning large, eukaryotic genomes
D)   when the DNA inserts to be used for cloning are very small
E)   when sequencing small proteinsF) A) and B)
G) C) and D)

Question 8

Which of the following blotting techniques allows a researcher to determine the number of copies of a gene within the genome of an organism?

Accepted Answer

A)   Western blotting
B)   colony hybridization
C)   Northern blotting
D)   Southern blotting
E)   Eastern blottingF) A) and E)
G) None of the above

Question 9

If a large protein is run on a gel slab and subjected to electrophoresis, one would expect to find its band towards the top of the gel.

Accepted Answer

A) True 
 B)False

Question 10

What is required for a group of clones to be considered a contig?

Accepted Answer

A)   The clones need to have nonoverlapping regions of DNA.
B)   The clones should contain identical regions of DNA.
C)   The clones should contain different regions of DNA.
D)   The clones should have overlapping regions of DNA.
E)   The clones should have identical, nonoverlapping regions of DNA.F) A) and C)
G) None of the above

Question 11

Which of the following pollutants could be reduced with the use of bioremediation?

Accepted Answer

A)   heavy metals
B)   petroleum hydrocarbons
C)   halogenated organic compounds
D)   pesticides
E)   All of the choices are correct.F) C) and D)
G) B) and D)

Question 12

What can be inferred from an organism that has undergone a gene knockout?

Accepted Answer

A)   The GMO is a heterozygote.
B)   The GMO is a homozygote.
C)   The cloned gene carries a mutation.
D)   The GMO is a heterozygote and the cloned gene carries a mutation.
E)   The GMO is a homozygote and the cloned gene carries a mutation.F) B) and C)
G) C) and D)

Question 13

The entire collection of a species' proteins is known as its

Accepted Answer

A)   genome.
B)   proteome.
C)   metabolome.
D)   microsome.
E)   transcriptosome.F) A) and B)
G) A) and C)

Question 14

What type of science is a researcher performing if she were conducting experiments to try and map the location of a gene on a particular chromosome?

Accepted Answer

A)   functional genomics
B)   structural genomics
C)   proteomics
D)   functional genomics and proteomics
E)   structural genomics and proteomicsF) A) and E)
G) B) and E)

Question 15

Which of the following is TRUE regarding the step in gene cloning when bacterial cells take up recombinant vector?

Accepted Answer

A)   The process is known as transformation when a viral vector is used.
B)   The process is known as transfection when a plasmid vector is used.
C)   The cell is treated with agents that render it impermeable to the plasmid.
D)   The cell is treated with agents that render it permeable to the plasmid.
E)   Most cells will successfully take up one plasmid.F) C) and E)
G) A) and D)

Question 16

Which would be TRUE of comparing the DNA fingerprints from hair samples of identical twins?

Accepted Answer

A)   Some bands match.
B)   No bands match.
C)   Every band matches.
D)   Exactly 50% of the bands match.
E)   Most bands match.F) B) and D)
G) A) and B)

Exam 20: Genetic Technology

Which of the following blotting techniques allows a researcher to determine the number of copies of a gene within the genome of an organism?

Correct Answer
verified

Which of the following sequence pairs is a palindrome?

Correct Answer
verified

If a large protein is run on a gel slab and subjected to electrophoresis, one would expect to find its band towards the top of the gel.

Correct Answer
verified

What is the purpose of a selectable marker such as the antibiotic-resistance gene ampR?

Correct Answer
verified

What is required for a group of clones to be considered a contig?

Correct Answer
verified

What can be inferred from an organism that has undergone a gene knockout?

Correct Answer
verified

Under what circumstances would a molecular geneticist need to use a bacterial artificial chromosome (BAC) ?

Correct Answer
verified

What method must be performed to produce enough DNA for sequencing?

Correct Answer
verified

Which would be TRUE of comparing the DNA fingerprints from hair samples of identical twins?

Correct Answer
verified

A researcher wants to clone a specific gene of interest. Why would he/she choose a viral vector for introducing the gene of interest into a host cell?

Correct Answer
verified

Which of the following acts as a carrier of foreign DNA and is needed to clone a gene?

Correct Answer
verified

A plasmid vector and chromosomal DNA are treated separately with the same restriction enzyme. Which of the following might occur if the digested plasmid and chromosomal DNA were incubated together?

Correct Answer
verified

The entire collection of a species' proteins is known as its

Correct Answer
verified

Which of the following is TRUE regarding the step in gene cloning when bacterial cells take up recombinant vector?

Correct Answer
verified

What type of science is a researcher performing if she were conducting experiments to try and map the location of a gene on a particular chromosome?

Correct Answer
verified

Which of the following pollutants could be reduced with the use of bioremediation?

Correct Answer
verified

Exam 20: Genetic Technology

Which of the following blotting techniques allows a researcher to determine the number of copies of a gene within the genome of an organism?

Correct AnswerverifiedShow Answer

Which of the following sequence pairs is a palindrome?

Correct AnswerverifiedShow Answer

If a large protein is run on a gel slab and subjected to electrophoresis, one would expect to find its band towards the top of the gel.

Correct AnswerverifiedShow Answer

What is the purpose of a selectable marker such as the antibiotic-resistance gene ampR?

Correct AnswerverifiedShow Answer

What is required for a group of clones to be considered a contig?

Correct AnswerverifiedShow Answer

What can be inferred from an organism that has undergone a gene knockout?

Correct AnswerverifiedShow Answer

Under what circumstances would a molecular geneticist need to use a bacterial artificial chromosome (BAC) ?

Correct AnswerverifiedShow Answer

What method must be performed to produce enough DNA for sequencing?

Correct AnswerverifiedShow Answer

Which would be TRUE of comparing the DNA fingerprints from hair samples of identical twins?

Correct AnswerverifiedShow Answer

A researcher wants to clone a specific gene of interest. Why would he/she choose a viral vector for introducing the gene of interest into a host cell?

Correct AnswerverifiedShow Answer

Which of the following acts as a carrier of foreign DNA and is needed to clone a gene?

Correct AnswerverifiedShow Answer

A plasmid vector and chromosomal DNA are treated separately with the same restriction enzyme. Which of the following might occur if the digested plasmid and chromosomal DNA were incubated together?

Correct AnswerverifiedShow Answer

The entire collection of a species' proteins is known as its

Correct AnswerverifiedShow Answer

Which of the following is TRUE regarding the step in gene cloning when bacterial cells take up recombinant vector?

Correct AnswerverifiedShow Answer

What type of science is a researcher performing if she were conducting experiments to try and map the location of a gene on a particular chromosome?

Correct AnswerverifiedShow Answer

Which of the following pollutants could be reduced with the use of bioremediation?

Correct AnswerverifiedShow Answer

Correct Answer
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Correct Answer
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