Question 1

Which is not true about the lac repressor?

Accepted Answer

A)  Its gene is part of the lac operon and is transcribed along with lacZ,lacA and lacY.
B)  When it binds to DNA,it forms a loop-type structure.
C)  It likely blocks both RNA polymerase binding as well as transcription initiation.
D)  It binds to DNA by interaction of an α-helix with the major groove.E) B) and D)
F) A) and B)

Question 2

TFIIB can bind to DNA in the absence of TFIID.

Accepted Answer

A) True 
 B)False

Question 3

Which has its primary transcript produced in the nucleolus?

Accepted Answer

A)  tRNA
B)  mRNA
C)  rRNA
D)  sRNAE) A) and B)
F) All of the above

Question 4

The unwinding of DNA at the initiation site is an example of a conformational change.

Accepted Answer

A) True 
 B)False

Question 5

What does it mean to say that the most common type of promoter in E.coli is bipartite?

Accepted Answer

A)  The promoter region is divided into two parts which flank the sequence for the gene.
B)  There are two separate regions of sequence similarity in the promoter.
C)  The sequence of the promoter has dyad symmetry.
D)  The promoter must bind two RNA polymerase holoenzymes to transcribe the gene.E) All of the above
F) A) and B)

Question 6

Which is not a feature of eukaryotic mRNA processing?

Accepted Answer

A)  Addition of a 5'-5' triphosphate linkage to GTP.
B)  Methylation of the terminal CCA triad.
C)  Addition of a polyA tail to the 3' end.
D)  Removal of intron sequences.E) All of the above
F) A) and B)

Question 7

An operon is ________.

Accepted Answer

A)  a set of genes whose transcription is controlled by a single promoter
B)  the site of repressor binding
C)  a protein that activates the transcription of a whole class of genes
D)  a multisubunit complex responsible for the splicing of exonsE) A) and B)
F) A) and C)

Question 8

The same RNA polymerase that transcribes the ribosomal RNA transcribes all mitochondrial genes.

Accepted Answer

A) True 
 B)False

Question 9

RNase D is an endonuclease that adds CCA to the 3' end of tRNA.

Accepted Answer

A) True 
 B)False

Question 10

A transcription complex does not form when one or more proteins bind to the promoter sequence and to RNA polymerase in eukaryotes.

Accepted Answer

A) True 
 B)False

Question 11

Which is true about the GTP cap on mRNA?

Accepted Answer

A)  The 5' carbon of the GTP sugar is connected via phosphates to the 5' end of the mRNA.
B)  The 5' carbon of the GTP sugar is connected via phosphates to the 3' end of the mRNA.
C)  The 3' carbon of the GTP sugar is connected via phosphates to the 5' end of the mRNA.
D)  The 3' carbon of the GTP sugar is connected via phosphates to the 3' end of the mRNA.E) All of the above
F) A) and D)

Question 12

Which of the following is an incorrect association between transcription factors and their characteristics?

Accepted Answer

A)  TFIIB : interacts with RNA polymerase II.
B)  SP1: binds to GC-rich sequence.
C)  TFIIH: couples transcription to DNA repair.
D)  TFIIE : recognizes the core sequence CCAAT.
E)  All of the above.F) B) and D)
G) All of the above

Question 13

Which statement is false about operons that have the feature of catabolic repression?

Accepted Answer

A)  Several different multisubunit repressors can block the transcription of such an operon.
B)  Operons with catabolic repression have characteristically weak promoters.
C)  Their transcription is lowered in the presence of glucose.
D)  Transcription of such an operon is increased as the concentration of cAMP increases.E) A) and C)
F) None of the above

Question 14

RNA polymerase requires a high-precision fit when catalyzing the formation of a new phosphodiester linkage.

Accepted Answer

A) True 
 B)False

Question 15

Which statement is false concerning the general transcription factor,TFIID?

Accepted Answer

A)  It binds to the promoter.
B)  Its TBP subunit binds to the region containing the TATA box.
C)  It interacts with TFIIA in the presence of DNA.
D)  It is the RNA polymerase II initiation factor.
E)  None of the above.F) B) and C)
G) B) and E)

Question 16

Positive regulation of a gene requires ________.

Accepted Answer

A)  an activator protein
B)  positive cooperativity
C)  an allosteric inducer
D)  the conversion of ATP to ADP and inorganic phosphateE) B) and D)
F) A) and B)

Question 17

Protein X is found to bind to a certain gene's promoter only when substance A is not present.The gene can be transcribed in the presence of both X and A.It is not transcribed in the presence of X alone.Protein X is a(n) ________.Substance A is a(n) ________.

Accepted Answer

A)  repressor;inducer
B)  repressor;corepressor
C)  activator;inducer
D)  activator;corepressorE) A) and B)
F) All of the above

Question 18

Which of the following is true about transcription?

Accepted Answer

A)  Promoters are regions of DNA which serve as transcription initiation sites.
B)  Initiation is the rate-limiting step in transcription.
C)  Each gene,in eukaryotic cells,usually has its own promoter.
D)  All of the above.E) A) and D)
F) All of the above

Question 19

Transcription sometimes stops near pause sites because these are regions

Accepted Answer

A)  rich in GC base pairs.
B)  rich in AT base pairs.
C)  without palindromic sequences.
D)  where Nus A is not present.E) None of the above
F) B) and D)

Question 20

Which statement is false about the utilization of lactose as a carbon source by E.coli?

Accepted Answer

A)  In the presence of both glucose and lactose,the enzymes necessary for lactose catabolism are produced only in limited amounts.
B)  Lactose is the inducer for the enzymes responsible for its own catabolism.
C)  In the absence of lactose,the repressor binds to a single site upstream of the lac genes.
D)  The catabolic enzymes are encoded in a three-gene operon controlled by a single promoter.E) B) and C)
F) None of the above

Exam 21: Transcription and RNA Processing

An operon is ________.

Correct AnswerverifiedShow Answer

RNase D is an endonuclease that adds CCA to the 3' end of tRNA.

Correct AnswerverifiedShow Answer

Which is not a feature of eukaryotic mRNA processing?

Correct AnswerverifiedShow Answer

TFIIB can bind to DNA in the absence of TFIID.

Correct AnswerverifiedShow Answer

Which of the following is true about transcription?

Correct AnswerverifiedShow Answer

RNA polymerase requires a high-precision fit when catalyzing the formation of a new phosphodiester linkage.

Correct AnswerverifiedShow Answer

Positive regulation of a gene requires ________.

Correct AnswerverifiedShow Answer

Which has its primary transcript produced in the nucleolus?

Correct AnswerverifiedShow Answer

Which is true about the GTP cap on mRNA?

Correct AnswerverifiedShow Answer

Which statement is false about the utilization of lactose as a carbon source by E.coli?

Correct AnswerverifiedShow Answer

Which is not true about the lac repressor?

Correct AnswerverifiedShow Answer

What does it mean to say that the most common type of promoter in E.coli is bipartite?

Correct AnswerverifiedShow Answer

Which of the following is an incorrect association between transcription factors and their characteristics?

Correct AnswerverifiedShow Answer

The same RNA polymerase that transcribes the ribosomal RNA transcribes all mitochondrial genes.

Correct AnswerverifiedShow Answer

Protein X is found to bind to a certain gene's promoter only when substance A is not present.The gene can be transcribed in the presence of both X and A.It is not transcribed in the presence of X alone.Protein X is a(n) ________.Substance A is a(n) ________.

Correct AnswerverifiedShow Answer

Which statement is false about operons that have the feature of catabolic repression?

Correct AnswerverifiedShow Answer

The unwinding of DNA at the initiation site is an example of a conformational change.

Correct AnswerverifiedShow Answer

A transcription complex does not form when one or more proteins bind to the promoter sequence and to RNA polymerase in eukaryotes.

Correct AnswerverifiedShow Answer

Which statement is false concerning the general transcription factor,TFIID?

Correct AnswerverifiedShow Answer

Transcription sometimes stops near pause sites because these are regions

Correct AnswerverifiedShow Answer

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Protein X is found to bind to a certain gene's promoter only when substance A is not present.The gene can be transcribed in the presence of both X and A.It is not transcribed in the presence of X alone.Protein X is a(n) .Substance A is a(n) .

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