Question 1

Specimen collection

Accepted Answer

A)  does not require special handling.
B)  must be done under sterile conditions.
C)  is always done by a medical professional.
D)  must utilize aseptic techniques.E) A) and C)
F) B) and C)

Question 2

You are the supervisor of a new reference microbiology laboratory, which will receive clinical specimens for identification of bacterial pathogens.When choosing the types of media to order for culture and isolation procedures in your laboratory, what question(s)  do you need to answer?

Accepted Answer

A)  What type of bacteria will you be culturing?
B)  What are the growth factors required by the bacteria that you will be identifying?
C)  What kind of microbiota will the patient specimens contain?
D)  All statements are correct.E) A) and D)
F) A) and C)

Question 3

The testing method involving the separation of serum proteins by electrophoresis, their subsequent transfer to special filters, and then probing with patient sera is called

Accepted Answer

A)  the Western blot.
B)  immunelectrophoresis.
C)  a radioimmunoassay (RI
A)  .
D)  Ouchterlony double diffusion.
E)  the Quellung test.F) A) and B)
G) All of the above

Question 4

The property of a test to detect even small amounts of antibodies or antigens that are test targets is ______.

Accepted Answer

A)  cross-reaction
B)  agglutination
C)  precipitation
D)  specificity
E)  sensitivityF) A) and B)
G) A) and C)

Question 5

ELISA tests use a fluorescent dye as a label to trace antigen-antibody reactions.

Accepted Answer

A) True 
 B)False

Question 6

Staphylococcus aureus can be identified by mixing the organism with specific antibody and visually recognizing a clumping reaction between the antigen and the antibody.This is a(n)  _______ test.

Accepted Answer

A)  microarray
B)  ELISA
C)  agglutination
D)  biochemicalE) A) and C)
F) A) and B)

Question 7

You place an inoculum of your bacterium on a glass slide and add a drop of hydrogen peroxide reagent.Promptly you see bubbles appear, indicating the presence of a particular enzyme that the bacterium produces.What type of identification test does this exemplify?

Accepted Answer

A)  Precipitation test
B)  Immunochromatography
C)  Microarray
D)  Biochemical testE) C) and D)
F) All of the above

Question 8

Which test cannot be used for identification of a person's HIV status?

Accepted Answer

A)  Immunochromatographic testing
B)  Biochemical tests
C)  Western Blot
D)  ELISAE) B) and D)
F) B) and C)

Question 9

Which of the following is not a phenotypic method of identification?

Accepted Answer

A)  Antibody response
B)  Acid-fast reaction
C)  Gram stain reaction
D)  Endospore production
E)  MorphologyF) B) and E)
G) A) and E)

Question 10

It is necessary to do lab tests to diagnose all diseases.

Accepted Answer

A) True 
 B)False

Question 11

Precipitation tests involve all of the following except

Accepted Answer

A)  a cloudy or opaque zone developing where antigen and antibody react.
B)  they rely on formation of visible clumps for detection.
C)  they can be done in a test tube by carefully adding antiserum over antigen solution.
D)  they include the VDRL test for syphilis.
E)  they are often performed in agar gels.F) C) and D)
G) B) and D)

Question 12

Antibody testing requires

Accepted Answer

A)  only a known antigen.
B)  only a known antibody.
C)  both a known antigen and a known antibody.
D)  either a known antigen or a known antibody.E) C) and D)
F) B) and C)

Question 13

The polymerase chain reaction requires endonuclease to cut samples of DNA.

Accepted Answer

A) True 
 B)False

Question 14

Serological testing always involves reactions between specific antibody and antigen.

Accepted Answer

A) True 
 B)False

Question 15

Which test is especially good for bacteria that are not readily cultivated in the lab?

Accepted Answer

A)  Gram stain
B)  Phage test
C)  Direct antigen test
D)  Direct fluorescence antibody (DF
A)   test
E)  Dichotomous key analysisF) A) and D)
G) B) and E)

Question 16

The primary advantage of genotypic analysis for identification is that

Accepted Answer

A)  it is less expensive than other methods.
B)  culturing of the organism is not required.
C)  it is easier than any other method.
D)  it is widely available.E) None of the above
F) A) and B)

Question 17

Which sample is not collected by sterile needle aspiration?

Accepted Answer

A)  Blood
B)  Urine
C)  Cerebrospinal fluid
D)  Tissue fluids
E)  All of the choices are collected by sterile needle aspiration.F) B) and E)
G) A) and E)

Question 18

Phage typing is useful in identifying ______.

Accepted Answer

A)  Mycobacterium leprae
B)  Streptococcus pyogenes
C)  Clostridium
D)  Treponema pallidum
E)  SalmonellaF) C) and D)
G) C) and E)

Question 19

Titer is the amount of _____ in serum.

Accepted Answer

A)  antibody
B)  antigen
C)  WBC
D)  memory cells
E)  complementF) B) and C)
G) A) and B)

Question 20

A positive serological test for tuberculosis indicates that the patient

Accepted Answer

A)  has active tuberculosis.
B)  is an asymptomatic carrier of tuberculosis.
C)  is immune to tuberculosis.
D)  has been exposed to tuberculosis.E) A) and C)
F) B) and D)

Exam 17: Diagnosing Infections

The property of a test to detect even small amounts of antibodies or antigens that are test targets is ______.

Correct AnswerverifiedShow Answer

Serological testing always involves reactions between specific antibody and antigen.

Correct AnswerverifiedShow Answer

You place an inoculum of your bacterium on a glass slide and add a drop of hydrogen peroxide reagent.Promptly you see bubbles appear, indicating the presence of a particular enzyme that the bacterium produces.What type of identification test does this exemplify?

Correct AnswerverifiedShow Answer

Antibody testing requires

Correct AnswerverifiedShow Answer

Precipitation tests involve all of the following except

Correct AnswerverifiedShow Answer

Phage typing is useful in identifying ______.

Correct AnswerverifiedShow Answer

Which of the following is not a phenotypic method of identification?

Correct AnswerverifiedShow Answer

Which test is especially good for bacteria that are not readily cultivated in the lab?

Correct AnswerverifiedShow Answer

A positive serological test for tuberculosis indicates that the patient

Correct AnswerverifiedShow Answer

Which test cannot be used for identification of a person's HIV status?

Correct AnswerverifiedShow Answer

It is necessary to do lab tests to diagnose all diseases.

Correct AnswerverifiedShow Answer

The testing method involving the separation of serum proteins by electrophoresis, their subsequent transfer to special filters, and then probing with patient sera is called

Correct AnswerverifiedShow Answer

Staphylococcus aureus can be identified by mixing the organism with specific antibody and visually recognizing a clumping reaction between the antigen and the antibody.This is a(n) _______ test.

Correct AnswerverifiedShow Answer

You are the supervisor of a new reference microbiology laboratory, which will receive clinical specimens for identification of bacterial pathogens.When choosing the types of media to order for culture and isolation procedures in your laboratory, what question(s) do you need to answer?

Correct AnswerverifiedShow Answer

Specimen collection

Correct AnswerverifiedShow Answer

The primary advantage of genotypic analysis for identification is that

Correct AnswerverifiedShow Answer

Which sample is not collected by sterile needle aspiration?

Correct AnswerverifiedShow Answer

The polymerase chain reaction requires endonuclease to cut samples of DNA.

Correct AnswerverifiedShow Answer

ELISA tests use a fluorescent dye as a label to trace antigen-antibody reactions.

Correct AnswerverifiedShow Answer

Titer is the amount of _____ in serum.

Correct AnswerverifiedShow Answer

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