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A commercially significant human protein now produced in bacteria is


A) hemoglobin.
B) gamma globulins.
C) AZT.
D) human insulin.
E) HIV vaccinE.

F) C) and D)
G) A) and B)

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All of the following involve molecular biology techniques except


A) cutting and rearranging the DNA.
B) using restriction enzymes to cut specific sequences of DNA.
C) cloning the genes into the host organism.
D) using any cell as a vector.

E) B) and C)
F) None of the above

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An insecticidal protein has been discovered in a bacterium known as


A) Escherichia coli.
B) Agrobacterium tumefaciens.
C) Bacillus thuringinensis.
D) Aspergillus sp.

E) All of the above
F) A) and C)

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Plant scientists have successfully engineered some broadleaf crop plants to be resistant to the application of glyphosate.Glyphosate works by


A) inhibiting an enzyme known as RNA polymerase,which is required for aromatic amino acid production.
B) inhibiting an enzyme known as DNA polymerase II,which is required for aromatic amino acid production.
C) inhibiting an enzyme known as EPSP synthetase,which is required for aromatic amino acid production.
D) inhibiting an enzyme known as EPSP synthetase,which is required for uncoupling the mRNA from ribosomes during protein synthesis.

E) B) and D)
F) A) and D)

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The polymerase chain reaction (PCR) includes all of the following steps except


A) denaturation of primers and the DNA fragment to be amplified.
B) anealing of primers to the complementary sequences on the DNA.
C) primer extension with DNA polymerase.
D) cycle repetition.
E) addition of new Taq polymerase after each cyclE.

F) A) and B)
G) C) and D)

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A plasmid used to carry genes into crop plants is


A) Ti.
B) glyphosphate.
C) somatotropin.
D) recombinant DNA.
E) Roundup.

F) A) and E)
G) B) and E)

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Automated enzymatic sequencing employs


A) different colored fluorescent dyes conjugated to dideoxy terminators and capillary gel electrophoresis.
B) radioactively-labeled dideoxy terminators and capillary gel electrophoresis.
C) different colored fluorescent dyes conjugated to dideoxy terminators and slab gel electrophoresis.
D) radioactively-labeled dideoxy terminators and slab gel electrophoresis.

E) All of the above
F) None of the above

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The most recent techniques developed in the biological sciences allow the manipulation of DNA with the ultimate goal of intervening directly with the _______ fate of organisms.


A) genetic
B) developmental
C) adult
D) mitotic

E) A) and B)
F) All of the above

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Most genetic engineering experiments include four stages.Which of the following is not one of them?


A) cleaving the source DNA
B) production of recombinant DNA
C) cloning copies of the recombinanats
D) screening the cloned copies for the desired gene
E) integration of the entire bacterial chromosome

F) A) and C)
G) B) and D)

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Viruses and bacterial plasmids are used as ______ to insert foreign DNA into host cells and create recombinant genomes.


A) clones
B) vectors
C) endonucleases
D) subunits

E) None of the above
F) B) and C)

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