Question 1

EcoRI and HindIII are

Accepted Answer

A)  palindromes.
B)  reverse transcriptase.
C)  restriction endonucleases.
D)  ligases.
E)  DNA polymerases.F) None of the above
G) B) and C)

Question 2

Replication of DNA begins at a(n) ____ rich area.

Accepted Answer

A)  guanine-cytosine.
B)  uracil-adenine.
C)  adenine-thymine.
D)  adenine-cytosine.
E)  guanine-adenine.F) C) and D)
G) A) and D)

Question 3

Split genes

Accepted Answer

A)  are common in bacteria and eukaryotes.
B)  only have exons initially transcribed to mRNA.
C)  have introns located only at the beginning and end of a coding region.
D)  use spliceosomes to excise introns and then join exons.
E)  All of the choices are correct.F) A) and B)
G) C) and D)

Question 4

The lactose repressor

Accepted Answer

A)  is transcribed with the structural lac genes.
B)  is activated by binding lactose.
C)  is inactivated by binding lactose.
D)  requires lactose for its transcription.
E)  None of the choices is correct.F) B) and C)
G) C) and D)

Question 5

The DNA of microorganisms is made up of subunits called

Accepted Answer

A)  histones.
B)  amino acids.
C)  nucleotides.
D)  mRNA.
E)  polymerases.F) A) and D)
G) A) and E)

Question 6

All mutations are detrimental to the cell.

Accepted Answer

A) True 
 B)False

Question 7

The primers in PCR are

Accepted Answer

A)  synthetic short strands of DNA (oligonucleotides) .
B)  bacterial enzymes.
C)  short RNA strands.
D)  DNA polymerases.
E)  reverse transcriptases.F) C) and E)
G) All of the above

Question 8

Bacterial conjugation involves

Accepted Answer

A)  bacteriophage carrying donor DNA to the recipient cell.
B)  a donor cell with a plasmid that synthesizes a pilus.
C)  naked DNA fragments from a lysed donor cell taken up by a recipient cell.
D)  new progeny cells with genes from two parent bacterial cells.
E)  None of the choices is correct.F) A) and E)
G) A) and D)

Question 9

If the wild-type DNA sequence reads THE CAT ATE THE BIG RAT,what type of mutation would change the sequence to THE CAT ATE THE BAG RAT?

Accepted Answer

A)  missense
B)  nonsense
C)  insertion
D)  deletion
E)  silentF) A) and B)
G) A) and C)

Question 10

Analysis of DNA fragments in gel electrophoresis involves

Accepted Answer

A)  larger fragments that move slowly and remain closer to the wells.
B)  DNA that has an overall negative charge and moves to the positive pole.
C)  DNA fragments that are stained to see them.
D)  an electric current through the gel that causes DNA fragments to migrate.
E)  All of the choices are correct.F) A) and D)
G) A) and C)

Question 11

DNA Polymerase III

Accepted Answer

A)  synthesizes primer.
B)  removes primer.
C)  joins Okazaki fragments.
D)  unzips the DNA helix.
E)  adds new bases and proofreads new DNA.F) B) and C)
G) C) and E)

Question 12

Lactose is a corepressor in the lac operon.

Accepted Answer

A) True 
 B)False

Question 13

The antiparallel arrangement within DNA molecules refers to

Accepted Answer

A)  each base bonding at the 1' position of the sugar.
B)  a purine always bonding to a pyrimidine.
C)  one helix strand that runs from the 5' to 3' direction and the other strand runs from the 3' to 5' direction.
D)  an original parent DNA strand and one newly synthesized DNA strand comprising a new DNA molecule.
E)  None of the choices is correct.F) A) and D)
G) B) and C)

Question 14

After three replication cycles in PCR,there will be a total of three double-stranded DNA molecules.

Accepted Answer

A) True 
 B)False

Question 15

A sequence of bases on a gene that does not code for protein is called a(n)

Accepted Answer

A)  promoter.
B)  operator.
C)  operon.
D)  exon.
E)  intron.F) B) and C)
G) None of the above

Question 16

The _____ is all of the genetic material of a cell.

Accepted Answer

A)  chromosome
B)  plasmid
C)  prophage
D)  genome
E)  proteomeF) A) and C)
G) A) and E)

Question 17

If you start with three double-stranded DNA fragments,after four cycles of PCR you will have ____ fragments.

Accepted Answer

A)  12
B)  24
C)  27
D)  48
E)  81F) B) and C)
G) A) and E)

Question 18

The most serious type of mutation is a

Accepted Answer

A)  point mutation.
B)  silent mutation.
C)  frame shift mutation.
D)  back mutation.
E)  All the choices are equally serious.F) B) and D)
G) A) and E)

Question 19

Which PCR step causes the denaturation of double-stranded DNA?

Accepted Answer

A)  add DNA polymerase and nucleotides at 72 ∘\circ∘ C
B)  cool DNA to between 50 ∘\circ∘ C and 65 ∘\circ∘ C
C)  add primers
D)  heat target DNA to 94 ∘\circ∘ C
E)  repeat the cycle of heating and cooling F) C) and D)
G) None of the above

Question 20

Please read the clinical scenario, and then answer the question that follow to become familiar with the traditional NCLEX question format.
  As an RN working in a research unit, you are responsible for data and specimen collection for multiple research studies. A patient is admitted as a participant in a study focused on the role of tRNA mutations in Alzheimer’s disease. The 78-year-old male is accompanied by his wife and daughter. Prior to obtaining the first blood specimen, you provide education regarding the importance of the study and the overall process of protein synthesis. 
-During protein synthesis,the mRNA codon,UAA,recruits which of the following tRNA molecules to the ribosomal complex?

Accepted Answer

A)  tRNA carrying methionine
B)  tRNA carrying proline
C)  tRNA carrying arginine
D)  No corresponding tRNA exists for this codon.E) B) and C)
F) A) and D)

Exam 8: Microbial Genetics and Genetic Engineering

Lactose is a corepressor in the lac operon.

Correct Answer
verified

Replication of DNA begins at a(n) ____ rich area.

Correct Answer
verified

The most serious type of mutation is a

Correct Answer
verified

The lactose repressor

Correct Answer
verified

EcoRI and HindIII are

Correct Answer
verified

Analysis of DNA fragments in gel electrophoresis involves

Correct Answer
verified

After three replication cycles in PCR,there will be a total of three double-stranded DNA molecules.

Correct Answer
verified

A sequence of bases on a gene that does not code for protein is called a(n)

Correct Answer
verified

Bacterial conjugation involves

Correct Answer
verified

If you start with three double-stranded DNA fragments,after four cycles of PCR you will have ____ fragments.

Correct Answer
verified

Split genes

Correct Answer
verified

Correct Answer
verified

If the wild-type DNA sequence reads THE CAT ATE THE BIG RAT,what type of mutation would change the sequence to THE CAT ATE THE BAG RAT?

Correct Answer
verified

Which PCR step causes the denaturation of double-stranded DNA?

Correct Answer
verified

The _____ is all of the genetic material of a cell.

Correct Answer
verified

The primers in PCR are

Correct Answer
verified

All mutations are detrimental to the cell.

Correct Answer
verified

DNA Polymerase III

Correct Answer
verified

The DNA of microorganisms is made up of subunits called

Correct Answer
verified

The antiparallel arrangement within DNA molecules refers to

Correct Answer
verified

Exam 8: Microbial Genetics and Genetic Engineering

Lactose is a corepressor in the lac operon.

Correct AnswerverifiedShow Answer

Replication of DNA begins at a(n) ____ rich area.

Correct AnswerverifiedShow Answer

The most serious type of mutation is a

Correct AnswerverifiedShow Answer

The lactose repressor

Correct AnswerverifiedShow Answer

EcoRI and HindIII are

Correct AnswerverifiedShow Answer

Analysis of DNA fragments in gel electrophoresis involves

Correct AnswerverifiedShow Answer

After three replication cycles in PCR,there will be a total of three double-stranded DNA molecules.

Correct AnswerverifiedShow Answer

A sequence of bases on a gene that does not code for protein is called a(n)

Correct AnswerverifiedShow Answer

Bacterial conjugation involves

Correct AnswerverifiedShow Answer

If you start with three double-stranded DNA fragments,after four cycles of PCR you will have ____ fragments.

Correct AnswerverifiedShow Answer

Split genes

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

If the wild-type DNA sequence reads THE CAT ATE THE BIG RAT,what type of mutation would change the sequence to THE CAT ATE THE BAG RAT?

Correct AnswerverifiedShow Answer

Which PCR step causes the denaturation of double-stranded DNA?

Correct AnswerverifiedShow Answer

The _____ is all of the genetic material of a cell.

Correct AnswerverifiedShow Answer

The primers in PCR are

Correct AnswerverifiedShow Answer

All mutations are detrimental to the cell.

Correct AnswerverifiedShow Answer

DNA Polymerase III

Correct AnswerverifiedShow Answer

The DNA of microorganisms is made up of subunits called

Correct AnswerverifiedShow Answer

The antiparallel arrangement within DNA molecules refers to

Correct AnswerverifiedShow Answer

Correct Answer
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Correct Answer
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Correct Answer
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