Question 1

ELISA tests use a fluorescent dye as a label to trace antigen-antibody reactions.

Accepted Answer

A) True 
 B)False

Question 2

When minute samples of DNA need to be genetically analyzed for identification purposes,which test can be effectively used?

Accepted Answer

A)  direct fluorescence antibody
B)  Widal
C)  Weil-Felix reaction
D)  PCR
E)  Western BlotF) A) and E)
G) B) and E)

Question 3

Both pulse-field gel electrophoresis and Western blotting utilize electrophoresis in their procedures.When comparing the two procedures,which statement is true?

Accepted Answer

A)  Pulse-field gel electrophoresis is used for genetic analysis.
B)  Pulse-field gel electrophoresis separates DNA fragments while Western blotting separates proteins.
C)  The presence of antibodies in a patient specimen can be identified in the Western Blot.
D)  Pulse-field gel electrophoresis is excellent for identifying minute genetic differences among strains of one species of an organism.
E)  All statements are true.F) C) and D)
G) B) and E)

Question 4

Viruses can be used to identify bacteria because of viral specificity for a host cell.This identification method is called:

Accepted Answer

A)  the ELISA.
B)  immunochromatography.
C)  probing.
D)  phage typing.E) C) and D)
F) B) and C)

Question 5

Urine and fecal specimens require

Accepted Answer

A)  sterile collection conditions.
B)  incubation in differential media.
C)  incubation in selective media.
D)  Both sterile collection conditions and incubation in differential media are correct.
E)  Both incubation in differential media and incubation in selective media are correct.F) C) and D)
G) B) and E)

Question 6

The ELISA is commonly used for diagnosing salmonellosis.

Accepted Answer

A) True 
 B)False

Question 7

Which sample is not collected by sterile needle aspiration?

Accepted Answer

A)  blood
B)  urine
C)  cerebrospinal fluid
D)  tissue fluids
E)  All of the choices are collected by sterile needle aspiration.F) B) and C)
G) C) and E)

Question 8

Which test compares the percentage of cytosine and guanine bases to adenine and thymine bases?

Accepted Answer

A)  rRNA sequencing
B)  electrophoresis
C)  G + C composition
D)  direct antigen testing
E)  DNA analysis with probesF) A) and B)
G) B) and D)

Question 9

What type of test affixes an antigen to an inert particle such as a latex bead?

Accepted Answer

A)  cross-reactions
B)  agglutination
C)  precipitation
D)  specificity
E)  sensitivityF) A) and E)
G) B) and E)

Question 10

In your microbiology lab,your class is identifying bacteria.You are using dichotomous keys to aid in the identification process using the data from a variety of biochemical tests.What problems do you foresee in this procedure,using the key?

Accepted Answer

A)  Dichotomous keys are too sophisticated to read properly,so you are worried that there will be a large degree of error in your identification.
B)  Dichotomous keys are not valid for identification.
C)  The dichotomous key does not contain information on your bacterium.
D)  If you misread a biochemical test or write down the wrong result,the key will likely take you to an alternative organsm that is not your unknown bacterium.E) B) and C)
F) A) and C)

Question 11

Which test is the confirming test for people who initially tested antibody-positive in the screening ELISA test?

Accepted Answer

A)  Ouchterlony double diffusion
B)  Western blot
C)  immunelectrophoresis
D)  radioimmunoassay (RI
A)  
E)  the Quellung testF) None of the above
G) All of the above

Question 12

The ELISA test has been used as the most common,rapid screening test for HIV infection.Its sensitivity is very high but its specificity is rather low (for an important identification test) .Which statement describes this accurately?

Accepted Answer

A)  Only certain kinds of instruments can be used to pick up the small amounts of fluorescence given off in the ELISA test.
B)  The test can identify the patient's antibody to HIV only when it is large quantities.
C)  The patient's specimen has to be handled in a certain way to maintain the integrity of the viral DNA.
D)  A false positive test result may develop due to the test antigens capturing normal antibodies.E) All of the above
F) B) and C)

Question 13

The testing method involving the separation of serum proteins by electrophoresis,their subsequent transfer to special filters,and then probing with patient sera is the:

Accepted Answer

A)  Ouchterlony double diffusion.
B)  Western blot.
C)  immunelectrophoresis.
D)  radioimmunoassay (RI
A)  .
E)  the Quellung test.F) A) and B)
G) C) and D)

Question 14

Biochemical tests include all of the following except

Accepted Answer

A)  antibiotic sensitivity tests.
B)  tests for the presence of specific enzymes.
C)  ribotyping tests.
D)  sugar fermentation tests.
E)  gas production tests.F) A) and B)
G) All of the above

Question 15

It is necessary to do lab tests to diagnose all diseases.

Accepted Answer

A) True 
 B)False

Question 16

Which test is especially good for bacteria that are not readily cultivated in the lab?

Accepted Answer

A)  Gram stain
B)  direct antigen test
C)  dichotomous key analysis
D)  direct fluorescence antibody (DF
A)  test
E)  phage testF) A) and E)
G) D) and E)

Question 17

Which of these tests is an in vivo test?

Accepted Answer

A)  tuberculin test
B)  Western Blot
C)  agglutination test
D)  PCRE) C) and D)
F) None of the above A

Question 18

Mycobacterium tuberculosis has traditionally been identified in patient samples using biochemical tests,and more recently through DNA probe analysis.In vivo testing can also be completed using the tuberculin skin test.
A more recent test that seems to be surpassing the others is the QuantiFERON-TB gold test.Blood samples are mixed with protein antigens of M.tuberculosis.After 16 to 24 hours of incubation,the amount of the cytokine interferon produced by the patientt's cells is measured.If the patient is infected with M.tuberculosis,their WBCs will release interferon in response to contact with the tuberculosis protein antigens.This test falls under which category of identification tests?

Accepted Answer

A)  microarray test
B)  immunologic test
C)  enzymatic test
D)  genotypic test
E)  DNA fingerprinting testF) A) and B)
G) None of the above B

Question 19

Nucleic acid testing is appropriate as a testing method when one is not sure what microorganisms are present in the specimen.

Accepted Answer

A) True 
 B)False  True

Question 20

Which test is used to identify antigens on red blood cells for blood typing?

Accepted Answer

A)  ELISA
B)  Western blot
C)  agglutination
D)  precipitation
E)  microarraysF) A) and B)
G) A) and C)

Exam 17: Diagnosing Infections

ELISA tests use a fluorescent dye as a label to trace antigen-antibody reactions.

Correct Answer
verified

When minute samples of DNA need to be genetically analyzed for identification purposes,which test can be effectively used?

Correct Answer
verified

Both pulse-field gel electrophoresis and Western blotting utilize electrophoresis in their procedures.When comparing the two procedures,which statement is true?

Correct Answer
verified

Viruses can be used to identify bacteria because of viral specificity for a host cell.This identification method is called:

Correct Answer
verified

Urine and fecal specimens require

Correct Answer
verified

The ELISA is commonly used for diagnosing salmonellosis.

Correct Answer
verified

Which sample is not collected by sterile needle aspiration?

Correct Answer
verified

Which test compares the percentage of cytosine and guanine bases to adenine and thymine bases?

Correct Answer
verified

What type of test affixes an antigen to an inert particle such as a latex bead?

Correct Answer
verified

In your microbiology lab,your class is identifying bacteria.You are using dichotomous keys to aid in the identification process using the data from a variety of biochemical tests.What problems do you foresee in this procedure,using the key?

Correct Answer
verified

Which test is the confirming test for people who initially tested antibody-positive in the screening ELISA test?

Correct Answer
verified

The ELISA test has been used as the most common,rapid screening test for HIV infection.Its sensitivity is very high but its specificity is rather low (for an important identification test) .Which statement describes this accurately?

Correct Answer
verified

The testing method involving the separation of serum proteins by electrophoresis,their subsequent transfer to special filters,and then probing with patient sera is the:

Correct Answer
verified

Biochemical tests include all of the following except

Correct Answer
verified

It is necessary to do lab tests to diagnose all diseases.

Correct Answer
verified

Which test is especially good for bacteria that are not readily cultivated in the lab?

Correct Answer
verified

Which of these tests is an in vivo test?

Correct Answer
verified
A

Correct Answer
verified
B

Nucleic acid testing is appropriate as a testing method when one is not sure what microorganisms are present in the specimen.

Correct Answer
verified
True

Which test is used to identify antigens on red blood cells for blood typing?

Correct Answer
verified

Exam 17: Diagnosing Infections

ELISA tests use a fluorescent dye as a label to trace antigen-antibody reactions.

Correct AnswerverifiedShow Answer

When minute samples of DNA need to be genetically analyzed for identification purposes,which test can be effectively used?

Correct AnswerverifiedShow Answer

Both pulse-field gel electrophoresis and Western blotting utilize electrophoresis in their procedures.When comparing the two procedures,which statement is true?

Correct AnswerverifiedShow Answer

Viruses can be used to identify bacteria because of viral specificity for a host cell.This identification method is called:

Correct AnswerverifiedShow Answer

Urine and fecal specimens require

Correct AnswerverifiedShow Answer

The ELISA is commonly used for diagnosing salmonellosis.

Correct AnswerverifiedShow Answer

Which sample is not collected by sterile needle aspiration?

Correct AnswerverifiedShow Answer

Which test compares the percentage of cytosine and guanine bases to adenine and thymine bases?

Correct AnswerverifiedShow Answer

What type of test affixes an antigen to an inert particle such as a latex bead?

Correct AnswerverifiedShow Answer

In your microbiology lab,your class is identifying bacteria.You are using dichotomous keys to aid in the identification process using the data from a variety of biochemical tests.What problems do you foresee in this procedure,using the key?

Correct AnswerverifiedShow Answer

Which test is the confirming test for people who initially tested antibody-positive in the screening ELISA test?

Correct AnswerverifiedShow Answer

The ELISA test has been used as the most common,rapid screening test for HIV infection.Its sensitivity is very high but its specificity is rather low (for an important identification test) .Which statement describes this accurately?

Correct AnswerverifiedShow Answer

The testing method involving the separation of serum proteins by electrophoresis,their subsequent transfer to special filters,and then probing with patient sera is the:

Correct AnswerverifiedShow Answer

Biochemical tests include all of the following except

Correct AnswerverifiedShow Answer

It is necessary to do lab tests to diagnose all diseases.

Correct AnswerverifiedShow Answer

Which test is especially good for bacteria that are not readily cultivated in the lab?

Correct AnswerverifiedShow Answer

Which of these tests is an in vivo test?

Correct AnswerverifiedA

Correct AnswerverifiedB

Nucleic acid testing is appropriate as a testing method when one is not sure what microorganisms are present in the specimen.

Correct AnswerverifiedTrue

Which test is used to identify antigens on red blood cells for blood typing?

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
A

Correct Answer
verified
B

Correct Answer
verified
True

Correct Answer
verified