Question 1

All of the following pertain to the fluorescence microscope except

Accepted Answer

A)  uses electrons to produce a specimen image.
B)  type of compound microscope.
C)  requires the use of dyes like acridine and fluorescein.
D)  commonly used to diagnose certain infections.
E)  requires an ultraviolet radiation source.F) None of the above
G) B) and D)

Question 2

The Gram staining procedure is best described as a/n __staining technique.

Accepted Answer

A)  selective
B)  differential
C)  negative
D)  simple
E)  specialF) A) and B)
G) A) and C)

Question 3

The three physical forms of laboratory media are

Accepted Answer

A)  solid,liquid,and gas.
B)  solid,semisolid,and liquid.
C)  streak plate,pour plate,and broth.
D)  aerobic,anaerobic,and microaerobic.E) A) and D)
F) All of the above

Question 4

When comparing the uses and benefits of the transmission electron microscope to the scanning electron microscope,you could say that:

Accepted Answer

A)  the transmission EM has to be used with dyes.
B)  the transmission EM is for external architecture of cells.
C)  the transmission EM is used for internal inspection of cells and subcellular structures.
D)  the transmission EM can be used with bacteria but not with viruses.E) B) and C)
F) A) and B)

Question 5

Which isolation technique is most effective for the majority of applications,and is most commonly used for colony isolation in the laboratory?

Accepted Answer

A)  pour plate
B)  streak plate
C)  spread plate
D)  loop dilution
E)  culture plateF) C) and E)
G) A) and B)

Question 6

Which type of media would be the best choice when shipping a sample of bacteria to a laboratory to be tested from a satellite office site?

Accepted Answer

A)  transport
B)  EMB
C)  blood
D)  thioglycollateE) All of the above
F) B) and C)

Question 7

Normal incubation temperatures range from 30$\circ$to 60$\circ$ C.

Accepted Answer

A) True 
 B)False

Question 8

A selective medium contains one or more substances that inhibit growth of certain microbes in order to facilitate the growth of other microbes.

Accepted Answer

A) True 
 B)False

Question 9

Which term refers to the microscope's ability to show two separate entities as separate and distinct?

Accepted Answer

A)  resolving power
B)  magnification,
C)  refraction
D)  magnificationE) All of the above
F) A) and D)

Question 10

A confocal scanning optical microscope

Accepted Answer

A)  uses a laser beam of light to form a specimen image.
B)  shows only the surface of the specimen.
C)  produces specimen images on electron micrographs.
D)  uses dyes that emit visible light when bombarded by ultraviolet rays.
E)  requires specimens to be stained.F) B) and E)
G) C) and D)

Question 11

Which of the following is not a benefit of agar as a solid medium?

Accepted Answer

A)  flexibility
B)  holds moisture
C)  can be inoculated and poured at a temperature that is not harmful
D)  solid at room temperature
E)  digested by most microbesF) A) and B)
G) A) and C)

Question 12

What do the Gram stain,acid-fast stain,and endospore stain have in common?

Accepted Answer

A)  used on a wet mount of the specimen
B)  use heat to force the dye into cell structures
C)  outcome based on the charge of the cell wall
D)  use a negative stain technique
E)  are differential stainsF) D) and E)
G) A) and E)

Question 13

Which method often results in colonies developing down throughout the agar along with some colonies on the surface?

Accepted Answer

A)  streak plate
B)  spread plate
C)  pour plate
D)  replica plateE) A) and B)
F) All of the above

Question 14

The bacterium Neisseria gonorrhoeae causes the sexually-transmitted infection gonorrhea.Although it can be isolated from the vagina,there are numerous normal biota in that location as well.In order to culture this pathogenic bacterium,one should use:

Accepted Answer

A)  enrichment media.
B)  selective media.
C)  refrigeration temperature for incubation.
D)  liquid media.E) B) and D)
F) None of the above

Question 15

All of the following are diameters of cells that would be resolved in a microscope with a limit of resolution of 0.2µm except

Accepted Answer

A)  0.2 µm.
B)  0.2 mm.
C)  0.1 µm.
D)  0.3 µm.
E)  2.0 µm.F) A) and E)
G) None of the above

Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

Which isolation technique is most effective for the majority of applications,and is most commonly used for colony isolation in the laboratory?

Correct Answer
verified

The three physical forms of laboratory media are

Correct Answer
verified

All of the following are diameters of cells that would be resolved in a microscope with a limit of resolution of 0.2µm except

Correct Answer
verified

Which type of media would be the best choice when shipping a sample of bacteria to a laboratory to be tested from a satellite office site?

Correct Answer
verified

A confocal scanning optical microscope

Correct Answer
verified

All of the following pertain to the fluorescence microscope except

Correct Answer
verified

Which method often results in colonies developing down throughout the agar along with some colonies on the surface?

Correct Answer
verified

The bacterium Neisseria gonorrhoeae causes the sexually-transmitted infection gonorrhea.Although it can be isolated from the vagina,there are numerous normal biota in that location as well.In order to culture this pathogenic bacterium,one should use:

Correct Answer
verified

A selective medium contains one or more substances that inhibit growth of certain microbes in order to facilitate the growth of other microbes.

Correct Answer
verified

Normal incubation temperatures range from 30^$\circ$to 60^$\circ$ C.

Correct Answer
verified

The Gram staining procedure is best described as a/n __staining technique.

Correct Answer
verified

Which of the following is not a benefit of agar as a solid medium?

Correct Answer
verified

What do the Gram stain,acid-fast stain,and endospore stain have in common?

Correct Answer
verified

Which term refers to the microscope's ability to show two separate entities as separate and distinct?

Correct Answer
verified

When comparing the uses and benefits of the transmission electron microscope to the scanning electron microscope,you could say that:

Correct Answer
verified

Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

Which isolation technique is most effective for the majority of applications,and is most commonly used for colony isolation in the laboratory?

Correct AnswerverifiedShow Answer

The three physical forms of laboratory media are

Correct AnswerverifiedShow Answer

All of the following are diameters of cells that would be resolved in a microscope with a limit of resolution of 0.2µm except

Correct AnswerverifiedShow Answer

Which type of media would be the best choice when shipping a sample of bacteria to a laboratory to be tested from a satellite office site?

Correct AnswerverifiedShow Answer

A confocal scanning optical microscope

Correct AnswerverifiedShow Answer

All of the following pertain to the fluorescence microscope except

Correct AnswerverifiedShow Answer

Which method often results in colonies developing down throughout the agar along with some colonies on the surface?

Correct AnswerverifiedShow Answer

The bacterium Neisseria gonorrhoeae causes the sexually-transmitted infection gonorrhea.Although it can be isolated from the vagina,there are numerous normal biota in that location as well.In order to culture this pathogenic bacterium,one should use:

Correct AnswerverifiedShow Answer

A selective medium contains one or more substances that inhibit growth of certain microbes in order to facilitate the growth of other microbes.

Correct AnswerverifiedShow Answer

Normal incubation temperatures range from 30 ∘\circ∘ to 60 ∘\circ∘ C.

Correct AnswerverifiedShow Answer

The Gram staining procedure is best described as a/n __staining technique.

Correct AnswerverifiedShow Answer

Which of the following is not a benefit of agar as a solid medium?

Correct AnswerverifiedShow Answer

What do the Gram stain,acid-fast stain,and endospore stain have in common?

Correct AnswerverifiedShow Answer

Which term refers to the microscope's ability to show two separate entities as separate and distinct?

Correct AnswerverifiedShow Answer

When comparing the uses and benefits of the transmission electron microscope to the scanning electron microscope,you could say that:

Correct AnswerverifiedShow Answer

Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Normal incubation temperatures range from 30^$\circ$to 60^$\circ$ C.

Correct Answer
verified

Correct Answer
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Correct Answer
verified

Correct Answer
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Correct Answer
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Correct Answer
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